Yanming Liu
Shandong Institute for Food and Drug Control, China
Title: Determination of L-carnitine in milk and dairy products by hydrophilic liquid chromatography tandem mass spectrometry
Biography
Biography: Yanming Liu
Abstract
An analytical method was developed for the determination of L-carnitine in milk and dairy products using hydrophilic interaction chromatography tandem mass spectrometry (HILIC-MS/MS). The samples were extracted with 2% acetic acid solution, and the protein was precipitated with acetonitrile subsequently. The separation of L-carnitine was carried out on an Acquity UPLC BEH HILIC column using ammonium acetate-acetonitrile as mobile phase. The quantitation analysis of target compound was performed under the multiple reaction monitoring (MRM) mode by the external standard method. A good linear relationship was obtained between peak area and concentration of L-carnitine in the range of 1-100ng/mL, with correlation coefficients more than 0.99 and limit of quantitation (LOQ) of 0.01 mg/Kg. LOQs of L-carnitine was 0.01 mg/Kg. The spiked recoveries were 96.0%-103.4%. The precision RSD was below 4.3%. The sample preparation was simple and rapid, the results were precise and sensitive. The developed method was suitable for the study of concentration of L-carnitine in milk and dairy product and provided the technical support for the infant formula.